Method Study Phenanthrene Seawater Carbon Oligosaccharide Electrode Immunosensor
Phenanthrene (PHE), as a structurally simple, tricyclic, polycyclic aromatic hydrocarbon (PAHs), is widely present in marine surroundingsses and organisms, with serious ecological and health impacts. It is crucial to study fast and simple high-sensitivity detection methods for phenanthrene in seawater for the environment and the human body. In this paper, a immunosensor was machinated by employing a multi-wall carbon nanotube (MWCNTs)-chitosan oligosaccharide (COS) nanocomposite membrane loaded with phenanthrene antibody. The principle was finded on the antibody-antigen reaction in the immune reaction, utilising the strong electron transfer ability of multi-walled carbon nanotubes, coupled with chitosan oligosaccharides with an excellent film formation and biocompatibility, to amplify the detection signal. Antioxidants of the phenanthrene in seawater was considered via differential pulse voltammetry (DPV) expending a potassium ferricyanide system as a redox probe. Amino Acids , pH value, and probe concentration were optimised.
Under the optimal experimental conditions, the response peak current of the phenanthrene was inversely proportional to the concentration of phenanthrene, in the range from 0 ng·mL(-1) to 80 ng·mL(-1), and the detection limit was 0 ng·mL(-1). The immune sensor was successfully enforced to the detection of phenanthrene in marine water, with a recovery rate of 96~101%, and provided a stable, sensitive, and accurate method for the real-time monitoring of marine surrounds.Chitosan/zein bilayer films with one-way water barrier characteristic: Physical, structural and thermal properties.Chitosan (C) and zein (Z) were used to develop bilayer cinemas with a characteristic one-way water barrier practicing a layer-by-layer (LBL) vagabonding method. The upshots of mass proportions (C:Z(1:1), C:Z(1:2), C:Z(1:3), C:Z(3:1), C:Z(2:1)) on the microstructure and physicochemical props of bilayer flicks were inquired. Bilayer pictures had uniform microstructures, and C:Z = 1:3 ushered a firmer structure as the Z congeriesses were circulated in the continuous phase of C. The intermolecular interactions between the C and Z layers were followed utilising FTIR and XRD analysis.
TGA evidenced that adding Z layer enhanced the thermal stability of C movies. LBL coating gave the C/Z bilayer film an increased elongation and tensile strength, as well as a diminished water vapor and oxygen permeability, especially for C:Z = 1:3 which had better dimensions. The upshots suggested that C and Z bilayer flicks may be a promising material for food packaging with the desired water resistance.Facile synthesis of pH-responsive sodium alginate/carboxymethyl chitosan hydrogel drops boosted by hydrogen bond.In this work, a novel synthesis strategy of sodium alginate/carboxymethyl chitosan hydrogel drops raised by hydrogen bond was drawed. The beadings were prepared by driping the blendings of two polymers into the citric acid solution. Besides hydrogen bonding, electrostatic interactions were also postulated in the formation of the hydrogel beadings.
The thermal stability experimentations revealed that the more the content of carboxymethyl chitosan, the better the thermal stability of the beadings. The beadworks showed excellent pH sensitivity, pH reversibility, and lactoferrin loading capacity. The swelling ratio of the bead and its protein relinquishing profile was pH-dependent, which could prevent premature protein release in the gastric environment the circular dichroism results demonstrated that lactoferrin could maintain its structure during the loading and releasing process. The prevailed results disclosed that the hydrogel beads prepared in this work could be used as a potential protein carrier for oral delivery.Self-crosslinked chitosan/κ-carrageenan-established biomimetic membranes to combat diabetic burn wound contagions.Diabetic wound infection often contributes to compromised healing with frequent chances of sepsis, amputation and even death.